The plant-based product is now available in Walmart's fresh meat section in 12-ounce packages. It is also available to purchase through Walmart Grocery Pickup & Delivery.
In an online statement, Impossible Foods CEO and Founder Dr. Patrick Brown said: "Impossible Foods' goal is to make the global food system sustainable. To do that, Impossible Burger has to be available everywhere people shop for meat.
"More Americans buy meat for their families at Walmart than anywhere else. We're confident that once Walmart customers try Impossible, they'll become enthusiastic advocates of our products and our mission."
At the beginning of the year, the Impossible Burger was only available in 150 grocery stores in California.
Now it is sold in more than 8,000 retail outlets across the U.S - as the company aims to expand its retail presence by 50 times in 2020 alone.
Last week, it debuted in popular grocery store Trader Joe's - hitting the shelves of 500 stores.
Impossible Foods' President Dennis Woodside said: "We're particularly excited about the launch of Impossible at Trader Joe's, a beloved institution with die-hard fans and a company known particularly for its great meat, cheese, and wine selection."