The meatless patty has been dubbed the 'Rebel Whopper' - and will be available in more than 50 stores in São Paulo from September 10.
According to Época Negócios, an advisory for the chain said in a statement: "The brand will assess public acceptance, then evaluate product expansion."
The '100 percent vegetable protein-based burgers' have been created by Marfig, one of the biggest hamburger producers in the world.
"We want to give consumers the power of choice. They are the ones who decide. The plant-based burgers will complement Marfrig's product portfolio and be distributed through all our market channels," said Eduardo Miron, CEO of Marfrig Global Foods, as the patty was released.
Earlier this month, the eatery launched its Impossible Whopper in all of its 7,200 US stores for a month-long trial.
The sandwich, which uses Impossible Food's plant-based patty, initially launched in 59 stores around St. Louis in Missouri on April 1. Since then, it has rolled out to eateries in Missouri, Miami, Alabama, Georgia, and the San Francisco Bay Area