In addition, almost 27,000 people have signed a petition calling on Twitch to ban Alinity aka Natalia Mogollon - who has more than 890,000 followers.
The Canadian gamer made headlines last week after throwing cat Milo over her shoulder last week, when he got in the way of her game. She also has a history of animal abuse, with a video from last year showing her spitting vodka, which can be toxic to cats, in Milo's mouth.
After the video of Alina throwing the cat came out, PETA tweeted Twitch saying: "Take a stand for cats and please remove Alinity from @Twitch immediately.
"She is using the platform to post animal abuse, which sends a dangerous message to anyone watching her videos. Cats are not props to abuse for more views."
Saskatoon Society for the Prevention of Cruelty to Animals confirmed it is 'investigating' the star's most recent act of animal cruelty, saying: "A formal cruelty complaint regarding this matter has been filed with our Animal Protection department and is currently under investigation."
The gamer published an apology on Twitter following the furore, saying: "Hey everyone, I understand the concern and I am sorry for my lapses in judgment.
"I shouldn't have gotten frustrated with Milo, but I dropped him on the floor behind my chair. I'm not that strong. The Vodka thing was well over a year ago but it was also a stupid thing to do.
"I also understand the desire to report me to pet authorities and encourage anyone to do so if they feel pets are being mistreated, by anyone, anywhere."
The gamer has since said she will not be banned by Twitch.